![ab cam duct ab cam duct](https://www.researchgate.net/profile/Adedamola-Olayanju/publication/283261893/figure/fig1/AS:314864695300098@1452081001437/UHRF1-is-over-expressed-in-pancreatic-cancer-A-Western-blot-of-UHRF1-in-a-panel-of_Q320.jpg)
Here, we demonstrated a mouse model to induce LG injury by the ligation of the lacrimal gland excretory duct. However, it has been still controversial that the duct ligation of the lacrimal gland excretory duct can cause lacrimal gland inflammation efficiently in an animal model. Obstruction of the fluid pathway itself has recently been recognized as a novel pathology to lead meibomian gland dysfunctions. ĭuct ligation of organs has been a conventional and promising method to induce organ dysfunctions including inflammation and fibrosis by obstructing the fluid pathway, blood vessels in the bile duct, and liver arteries.
![ab cam duct ab cam duct](https://www.abcam.com/ps/products/213/ab213149/Images/ab213149-459222-anti-hnf-1b-antibody-epr18644-13-immunohistochemistry-mouse-liver.jpg)
An establishment of animal models for an efficient LG injury has been required to elucidate pathological changes against persistent injuries to the LG. The reports indicated a regeneration capacity in the partial damaged LG. The single injection of IL-1, a cytokine, to the LG induced the inflammation partially and temporarily, and it caused reversible change after 7 days.
![ab cam duct ab cam duct](https://els-jbs-prod-cdn.jbs.elsevierhealth.com/cms/attachment/21eb270f-3148-4d37-8a36-5911897dd051/gr2_lrg.jpg)
To analyze the mechanism of tear decreasing after injury to the LG, several animal models, which could induce inflammation of the LG, have been proposed.
![ab cam duct ab cam duct](http://histosearch.com/imageupload/wp-content/uploads/2014/08/K10-E-1in6KHN1.jpg)
The myoepithelial cells that surround the acini help to secrete tear contents from acini in response to nerve stimulation. The acini express water channel proteins including aquaporin 5 (AQP5) for aqueous tear secretion. The LG is composed of various kinds of cells such as acinar, duct, and myoepithelial cells. A significance to treat DED based on the understanding on mechanism on pathological changes in the LG has increased because DED has become one of common eye diseases in elderly people that causes subjective symptoms including discomfort as well as objective signs such as foreign body sensation, ocular irritation, and blurred vision, which affect the quality of life. Dysfunction of the LG leads to dry eye disease (DED), an ocular surface disease characterized by insufficient tear production and damaged epithelium. The lacrimal gland (LG) is a secretory gland that secretes tear fluids to maintain the health of the ocular surface epithelium and protect the ocular surface from the external environment. This study successfully established an injury model to observe the tissue injury process of the lacrimal gland, and this model will be useful for analysis of the inflammation and proliferation mechanism in the future. We observed the process of injury and proliferation of the lacrimal gland and detected a population of lacrimal gland epithelial cells with proliferation potential which were also nestin-positive cells following duct ligation. Here, we demonstrated an injury model by ligating the main excretory duct of the lacrimal gland, which is a simple and stable way to clearly understand the mechanism of lacrimal gland inflammation. Inflammatory change in the damaged lacrimal gland has been reported however, a major challenge is to establish a simple animal model to observe the changes. The dry eye disease is becoming a popular public disease, for the number of patients is increasing, who have subjective symptom and loss of vision, which affect the quality of life. The dysfunction of the lacrimal glands causes dry eye disease due to a reduction in tear volume. The lacrimal gland secretes tear fluids to ocular surface, which plays an indispensable role in maintaining the health of the ocular epithelia and protecting the ocular surface from the external environment.